Hakim ÇELİK, Abdurrahim KOÇYİĞİT, Cahit BAĞCI, İsmail KOYUNCU, Ali Ziya KARAKILÇIK

Alüminyum Toksisitesine Maruz Bırakılmış İnsan Lenfosit Hücrelerinde Çörek Otu (Nigella sativa) Ekstraktının Anti-Genotoksik ve Anti-Sitotoksik Etkileri

Özet Amaç: Dünyadaki en yaygın üçüncü element olan alüminyum, vücutta biriktiğinde genotoksisite, sitotoksisite ve oksidatif strese neden olmaktadır. Ayrıca, çörek otunun timokinon ve fenolik bileşikler gibi güçlü antioksidanların kaynağı olduğu da bilinen bir gerçektir. Ancak çörek otunun, alüminyum toksisitesine karşı koruyucu etkiye sahip olup olmadığı henüz araştırılmamıştır. Bu nedenle, çalışmamızda alüminyum toksisitesine karşı çörek otunun anti-genotoksik ve anti-sitotoksik etkilerini in vitro hücre kültürü ortamında araştırmayı amaçladık. Materyal ve metod: İnsan lenfosit hücrelerinde, alüminyum toksisitesine karşı, çörek otunun antisitotoksik ve antigenotoksik etkileri, alkali tek hücre elektroforez yöntemi (comet assay), mikronükleus test, metil tiyazol tetrazolium (MTT) test ve DNA fragmantasyon analizi ile çalışıldı. Bulgular: Elde edilen bulgular, alüminyumun 100 μM'da genotoksik ve sitotoksik etkili olduğunu (p < 0,05) ve düşük konsantrasyonlarda (0,1-2 μg / mL) çörek otu ekstraktının alüminyum toksisitesine karşı anti-genotoksik ve anti-klastojenik etkilere sahip olduğunu göstermektedir (p < 0,05). Sonuç: Bu çalışma, alüminyumdan yapılmış malzemelerin kullanımının kısıtlanması ve alüminyum toksisitesini nötralize etmek için düşük miktarlarda çörek otunun tüketilmesi gerektiğini önermektedir.

Anahtar Kelimeler:

alüminyum toksisitesi, çörek otu, genotoksisite

Anti-Genotoxic and Anti-Cytotoxic Potential of Black Cumin Seed (Nigella sativa) Extract on Aluminum-exposed Human Lymphocyte Cells

AbstractBackground: The third most common element in the world, aluminum (Al), causes genotoxicity, cytotoxicity, and oxidative stress when its accumulation increases in the body. Conversely, Nigella sativa seeds (NSS) are a unique source of potent antioxidants, such as thymoquinone (TQ) and phenolic compounds. It is currently unclear if NSS have protective potential against the toxic efficiencies of Al. Therefore, this study aimed to assess the anti-genotoxic and anti-cytotoxic effects of the methanolic extract of Nigella sativa seeds (NSE) at different concentrations in human peripheral blood lymphocyte cells (PBLC) against Al toxicity in in vitro conditions. Methods: A comet assay, micronucleus test, DNA fragmentation analyses, and methyl-thiazol-tetrazolium (MTT) assay were used to evaluate the anti-genotoxic, anti-cytotoxic, and anti-clastogenic effects of NSE against the Al toxicity. Results: The obtained data showed that Al had genotoxic and cytotoxic effects at 100 μM of concentration (p < 0.05) and that NSE at low concentrations (0.1–2 μg/mL) had anti-genotoxic and anti-clastogenic effects against Al-toxicity (p < 0.05). It was also observed that NSE at or above 2 μg/mL of concentration had genotoxic effects and that NSE at or above 5 μg/mL of concentration had cytotoxic effects (p < 0.05). Conclusions: This study recommends that the use of materials made of Al should be restricted and that NSS should be consumed in low quantities to neutralize Al toxicity.

Keywords:

aluminum toxicity, nigella sativa, genotoxicity,

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