LISTERIA MONOCYTOGENES SPESİFİK FAJLARIN İZOLASYONU

Bu çalışmada Listeria monocytogenes'i enfekte eden litik fajların izole edilmesi, saflaştırılması ve konakçı etkinliklerinin belirlenmesi amaçlanmıştır. Toplamda 68 örnek taranmış ve dışkı, gıda işleme atık suları, balıkçılık atık suları ve balık örneklerinden 4 pozitif izolat elde edilmiştir. Faj varlığı bakımından taranan örneklerin % 5.88’inde L. monocytogenes fajı izole edilmiştir. Faj konakçı etkinliğinin belirlenmesi amacıyla, çift tabaka agar yöntemi kullanılmıştır. 11 L. monocytogenes suşu denenen dört faja karşı dirençli bulunurken, 24 suşta ise duyarlılık saptanmıştır. Dört fajın 24 L. monocytogenes suşuna karşı oluşturduğu faj plak çapı ise 0.4 ve 2.6 mm arasında değişmiştir. Hiçbir faj birbiri ile aynı konakçı etkinliği göstermemiştir.

Anahtar Kelimeler:

Listeria monocytogenes, Faj, İzolasyon, Konakçı Etkinliği, Duyarlılık

ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES

It was aimed to isolate, purify and determine host ranges of lytic phages that infect Listeria monocytogenes in this study. Out of 68 samples screened, 4 positive isolates were recovered from feces, food processing waste water, fisheries waste water, and fish samples. Recovery status of the L. monocytogenes phage was found to be 5.88%. To determine host ranges of phages, soft agar overlay plaque assay was used. While eleven L. monocytogenes strains showed resistant to all four isolated phages, 24 strains were sensitive. The plaque sizes of the 4 phages against 24 L. monocytogenes strains ranged from 0.4 and 2.6 mm. None of the phages had identical host ranges.

Keywords:

Listeria monocytogenes, Phage, Isolation, Host Range,

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