Türk Toplumunda MTHFR A1298C Polimorfizmi ile Oral Sküamoz Hücre Karsinom Gelişim Riski Arasındaki İlişkinin Araştırılması

DOI: 10.26650/experimed.2019.19009Amaç: Düşük yaşam süresi ile karakterizeolan ve çevresel faktörlerin yanı sıra genetik ve epigenetik değişimlerinkatkılarının bulunduğu oral sküamoz hücre karsinomu (OSHK) gün geçtikçeyükselen insidansıyla dikkati çekmektedir. Günümüze kadar, folat metabolizmasınınönemli enzimlerinden biri olan ve dolayısıyla DNA sentezi, metilasyonu ve tamirmekanizmalarında rol oynayan metilen tetrahidrofolat redüktaz (MTHFR) enzimineait genetik varyantlar ile kanser yatkınlığı arasındaki ilişkinin araştırıldığıbirçok çalışma yapılmıştır. Çalışmamızda, Türk popülasyonundaki MTHFR A1298Cvaryantları ile alkol ve/veya sigara kullanımı, cinsiyet, yaş veya klinikparametreler esasında oral sküamoz hücre karsinomu gelişim riskininaraştırılması hedeflenmiştir. Gereç ve Yöntem: MTHFR varyantları PCR-RFLPyöntemleri kullanılarak aynı yaş grubundaki 107 hasta ile 107 sağlıklı bireydeanaliz edilmiştir. Bulgular: Hasta ve kontrol grubu arasındaMTHFR A1298C genotip ve allel dağılımı bakımdan anlamlı bir fark eldeedilmemiştir. Sigara-içmeyen hastalarda, A1298 allel frekansı mutant allelegöre yüksek olup, bu alleli taşıyanlarda tümör çapının 4cm’den büyük olduğu vebu tümörlerin orta veya iyi diferansiasyona sahip olduğu tespit edilmiştir.Ancak bu durum sigara içen hastalarda tespit edilmemiştir. Sonuç: Çalışmamız sonucunda MTHFR A1298Cpolimorfizminin hastalık gelişiminde bir risk faktörü olmadığı, ancakhastalığın prognozunda özellikte mutant varyantın etkili olabileceği kanaatinevarılmıştır.Cite this article as: Küçükhüseyin Ö,Kayhan KB, Ünür M, Yılmaz Aydoğan H. Investigation Between MTHFR A1298Cpolymorphism and Oral Squamous Cell Carcinoma Risk in Turkish Population.Experimed 2019; 9(2): 44-52.

Investigation Between MTHFR A1298C polymorphism and Oral Squamous Cell Carcinoma Risk in Turkish Population

DOI: 10.26650/experimed.2019.19009Objective: Oral squamous cell carcinoma(OSCC) with its poor survival rates and rising number of incidences arisesthrough several etiological factors including environmental, genetic andepigenetic alterations. Several studies have established an association betweencancer susceptibility and polymorphisms of methylenetetrahydrofolate reductase(MTHFR), the key enzyme involved in folate metabolism and, therefore in DNAsynthesis, methylation and repair. The aim of the present study was toestablish any association between MTHFR A1298C variants and alcohol and/ortobacco consumption, gender or age in respect to clinical histopathologicalparameters, and the risk of OSCC development in the Turkish population. Material and Method: MTHFR A1298Cgenotyping in 107 OSCC patients and 107 cancer-free healthy controls wasperformed using the PCR-RFLP method. Results: The study groups were age-matchedwith higher frequencies in the male gender. In the patients group, thedistribution of MTHFR A1298C variants was not significant. Smoking was notfound to be a risk factor: in non-smokers the frequency of the MTHFR A1298allele was higher than the 1298C allele, and the A1298 allele carrierspossessed moderately or well differentiated tumors with a diameter of <4 cm.However, these associations were not detected in smokers. Conclusion: The present study alone did notdemonstrate any association between the MTHFR A1298C polymorphism and the riskof OSCC in the Turkish population, however the prognosis of OSCC may beinfluenced by MTHFR A1298C variants.Cite this article as: Küçükhüseyin Ö,Kayhan KB, Ünür M, Yılmaz Aydoğan H. Investigation Between MTHFR A1298Cpolymorphism and Oral Squamous Cell Carcinoma Risk in Turkish Population.Experimed 2019; 9(2): 44-52.

Kaynakça

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