İleri Patates Hatlarının Kuraklık Toleransının In Vitro Koşullarda Belirlenmesi

Araştırma bazı ileri patates hatlarında in vitro koşullarda PEG 6000 kullanılarak oluşturulan kuraklık stresinin etkisini belirlemek amacıyla yapılmıştır. Araştırma doku kültürü laboratuvarında 2016 yılı Ocak - Mayıs ayları arasında gerçekleştirilmiştir. Araştırma tesadüf blokları deneme desenine göre; MS0: PEG yok, P1: %3 PEG, P2= %10 PEG ve P3=%15 PEG olarak adlandırılan 4 PEG konsantrasyonu kullanılarak 3 tekerrürlü olarak yapılmıştır. Bitki boyu, bitki başına boğum sayısı ve bitki yaş ağırlığı gibi bitki özellikleri 20 ve 40 günlük kültür periyotlarında ölçülmüş ve değerlendirilmiştir. Genel olarak P1 (MS + %3 sukroz + 7 g/lagar + %3 PEG), P2 (MS + %3 sukroz + 7 g/lagar + %10 PEG) ve P3 (MS + %3 sukroz + 7 g/lagar + %15 PEG) ortamlarına göre tüm bitki özelliklerinde en yüksek değerler MSO (MS + %3 sukroz + 7 g/l agar ) ortamında elde edilmiştir. Araştırma sonuçlarına göre PEG konsantrasyonu artıkça bitki boyu, bitki başına boğum sayısı, bitki yaş ağırlığı ve bitki büyüme ve gelişmesi azalma göstermiştir. Araştırma sonucuna göre 13-80-44, 22-99-33 ve 12-69-39 ileri hatları PEG 6000 içeren in vitro koşullarda kuraklık toleransı yüksek hatlar olarak seçilmiştir.

Anahtar Kelimeler:

İleri hat, ıslah, kuraklık, patates

PDF

___

Albiski, F., S. Najla R. Sanoubar N. Alkabani and R. Murshed. 2013. In vitro screening of potato lines for drought tolerance. Physiology and Molecular Biology of Plants, 18(4): 315-321.
Charloq, E., Panjaitan and B.A. Sirait. 2012. Study of early screening of potato (Solanum tuberosum L.) as a result of drought stress (in vitro). Proceedings of the 2nd Annual International Conference Syiah Kuala University & The 8th IMT-GT Uninet Biosciences Conference Banda Aceh. 2(1): 403-405.
Dalla Costa, L., G. Delle Vedove G. Gianquintoi R. Giovanardi and A. Peressotti. 1997. Yield, water use efficiency and nitrogen uptake in potato: influence of drought stress. Potato Research, 40:19-34.
Deblonde, P.M.K and J.F. Ledent. 2001. Effects of moderate drought conditions on green leaf number, stem height, leaf length and tuber yield of potato cultivars. European Journal of Agronomy, 14: 31-41.
Deng, Z., J.F. Xu G.G. Duan J. Liu C.S. Bian W.F. Pand and L.P. Jin. 2014. Effect on growth indicators of 11 potato cultivars in vitro under PEG-8000 stress. Acta Agriculturae Boreali-Sinica, 29 (5): 99-106.
Eiasu, B.K., P. Soundy and P.S. Hammes. 2007. Response of potato (Solanum tuberosum) tuber yield components to gelpolymer soil amendments and irrigation regimes, New Zeland Journal of Crop Horticulture, 35: 25-31.
Ekanayake, I.J. and D.J. Midmore. 1992. Genotypic variation for root pulling resistance in potato and its relationship with yield under water-deficit stress. Euphytica, 61:43-53.
Freed, R., S.P. Einensmith S. Guetz D. Reicosky V.W. Smail and P. Wolberg. 1989. User’s Guide to MSTAT–C Analysis of Agronomic Research Experiments, Michigan State University USA.
Gopal, J. and K. Iwama. 2007. In vitro screening of potato against water-stress mediated through sorbitol and polyethylene glycol. Plant Cell Reports, 26: 693-700.
Hussain, M., M.A. Malik M. Farooq M.Y. Ashraf and M.A. Cheema. 2008. Improving drought tolerance by exogenous application of glycine-betaine and salicylic acid in sunflower. Journal of Agronomy and Crop Science, 194:193-199
Iwama, K. and J. Yamaguchi. 2006. Abiotic stresses, in: J. Gopal, S.M. Paul Khurana (Eds.), Handbook of Potato Production Improvement and Post Harvest Management, Food Product Press, New York. pp. 231-278.
Iwama, K. 2008. Physiology of the potato: new insights into root system and repercussions for crop management. Potato Research, 51: 333-353.
Jacomini, E., A. Bertani and Mapelli. 1988. Accumulation of polyethylene glycol 6000 and its effects on water content and carbohydrate level in water-stressed tomato plant, Canadian Journal of Botany, 66: 970-973.
Kaya, M.D., G. Okçu M. Atak Y. Çıkılı and Ö. Kolsarıcı. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24: 291-295.
Kızıloğlu, F.M., Ü. Sahin T. Tunç and S. Diler. 2006. The effect of deficit irrigation on potato evapotranspiration and tuber yield under cool season and semiarid climatic condition. Journal of Agronomy. 5: 284-288.
Kumar, S., R. Asrey and G. Mandal. 2007. Effect of differential irrigation regimes on potato (Solanum tuberosum) yield and post-harvest attributes, Indian Journal of Agricultural Science, 77: 366-368.
Lahlou, O., S. Ouattar and J.F. Ledent. 2003. The effect of drought and cultivar on growth parameters, yield and yield components of potato. Agronomie, 23: 257-268.
Manoj, K. and D. Uday. 2007. Gradient in vitro testing of tomato (Solanum lycopersicum) genotypes by inducing water deficit: a new approach to screen germplasm for drought tolerance. Asian Journal of Plant Science, 6: 934-940.
Mengü-Pamuk, G., S. Anaç ve E. Özçakal. 2011. Kuraklık yönetim stratejileri. Ege Üniversitesi Ziraat fakültesi Dergisi, 48(2): 175-181.
Monneveux, P., D.A. Ramíreza and M.T. Pino. 2013. Drought tolerance in potato (S. tuberosum L.) Can we learn from drought tolerance research in cereals ? Plant Science, 205-206: 76-86.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco cultures. Physiol. Plant. 5: 473-497.
Nistor, A., M. Cioloca N. Chiru and M. Popa. 2014. In vitro response to drought tolerance for different potato varieties. Analele Universităţii din Oradea, Fascicula Protecţia Mediului, 13: 257-262.
Qayyum, M. and K. Shoaib. 2013. Selection of potato (Solanum tuberosum L. cv. Cardinal) plantlets tolerant to in vitro salt and drought stress. Pakistan Journal of Biochemistry and Molecular Biology, 46(1): 37-41.
Pino, M.T., A. Ávila A. Molina Z. Jeknic and T.H.H. Chen. 2013. Enhanced in vitro drought tolerance of Solanum tuberosum and Solanum commersonii plants overexpressing the ScCBF1 gene. Cien. Inv. Agr., 40(1):171-184.
Renault, D. and W.W. Wallender. 2000. Nutritional water productivity and diets: from “crop per drop” towards “nutrition per drop”, Agricultural Water Management, 45: 275-296.
Schafleitner, R., R. Gutiérrez R. Espino A. Gaudin J. Pérez M. Martínez A. Domínguez L. Tincopa C. Alvarado G. Numberto and M. Bonierbale. 2007. Field screening for variation of drought tolerance in Solanum tuberosum L. by agronomical, physiological and genetic analysis. Potato Research, 50: 71-85.
Schittenhelm, S., H. Sourell and F. Lopmeierc. 2006. Drought resistance of potato cultivars with contrasting canopy architecture. European Journal of Agronomy, 24:193-202.
Soltys-Kalina, D., J. Plich, D. Strzelczyk-Żyta, J. Śliwka and W. Marczewski. 2016. The effect of drought stress on the leaf relative water content and tuber yield of a half-sib family of ‘Katahdin’-derived potato cultivars. Breeding Science, 66: 328-331.
Stark, J.C., S.L. Love, B.A. King, J.M. Marshall, W.H. Bohl and T. Salaiz. 2013 Potato cultivar response to seasonal drought patterns. American Journal of Potato Research, 90: 207-216.
Tábori, K.M., A. Hanász N.M. Drienyovszki L. Zsombik and j. Dobránszki. 2017. Study of osmotic stress tolerance of potato breeding clones in vitro shoot cultures. 20th EAPR Triennial Conference. 9-14 July 2017, Paris, France, p.98.
Tuberosa, R. 2012. Phenotyping for drought tolerance of crops in the genomics era. Front. Physiol. 3: 347.
Weisz, R., K. Kaminski and Z. Smilowitz. 1994. Water deficit effects on potato leaf growth and transpiration: utilizing fraction extractable soil water for comparison with other crops. American Journal of Potato Research, 71: 829-840.
Yaniv, Z. and E. Werker. 1983. Absorption and secretion of polyethylene glycol by Solanaceous plants, Journal of Experimental Botany, 34: 1577-1584.
Yuan, B.Z., S. Nishiyama and Y. Kang. 2003. Effects of different irrigation regimes on the growth and yield of drip-irrigated potato. Agricultural Water Management, 63: 153-167.