Fenolik İçeriği Yüksek Tarımsal Atıkların Hericium erinaceus ve Lentinula edodes Mantarlarının Üretiminde Kullanımı

Amaç: Bu çalışmada fenolik içeriği yüksek olan dört farklı tarımsal atığın Hericium erinaceus and Lentinula edodes mantarlarının üretiminde kullanımı araştırılmıştır.Materyal ve Metot: Bu amaçla, bu atıkların bahsedilen mantar türlerinin misel gelişimleri, verim ve biyolojik etkinlikleri (BE) etkileri karşılaştırmalı olarak değerlendirilmiştir. Meşe talaşı (OS) ana material olarak kullanılmış ve bu ortama %20 oranında üzüm posası (GP), yeşil ceviz kabuğu (GWH), zeytin pirinası (OPC) ve çay atıkları eklenerek mantar üretiminde kullanılmıştır. Denemeler 10 tekerrürlü olarak tesadüfi tarla parselleri deneme desenine göre planlanmıştır.Bulgular: Hem H. erinaceus hemde L. edodes’de en kısa misel gelişim süresi (22.4 ve 45.4 gün, sırasıyla) ve en yüksek verim (225.0 g kg-1 and 282.9 g kg-1,sırasıyla) OS ve GP kombinasyonlarında gözlenmiştir. H. erinaceus ve L. edodes mantarlarının BE’leri ise  sırası ile %15.2-64.3 ve %36.0-70.7 arasında değişmiştir. Sonuç: GP, TW ve OPC bu mantar türlerinin üretiminde alternative katkı materyali olarak kullanılabilirler. Fakat mantar üretiminde yeşil ceviz kabuğundan etkili ve karlı bir şekilde yararlanabilmemiz için ek çalışmalar yapılması gerekmektedir.

The use of phenolic-rich agricultural wastes for Hericium erinaceus and Lentinula edodes cultivation and its effect on yield performance

Objective: This research investigated that the use of four selected agro-waste materials rich in phenolic content on cultivation of Hericium erinaceus and Lentinula edodes mushrooms. Material and Methods: For this purpose, these wastes were comparatively evaluated regarding their suitability for mycelial growth, yield and biological efficiency (BE), of these mushroom species.  The oak sawdust (OS) basal medium was mixed with 20% grape pomace (GP), green walnut hull (GWH), olive press cake (OPC), and tea waste (TW) for the production of these species in factorial experiments based on a completely randomised design with ten replications. Results: For both H. erinaceus and L. edodes, the shortest spawn running time (22.4 and 45.4 days, respectively) and the highest yield (225.0 g kg-1 and 282.9 g kg-1, respectively) were found using GP. The BE of H. erinaceus and L. edodes were varied between 15.2-64.3% and 36.0-70.7% , respectively. Conclusion: GP, TW and OPC were seen as promising alternative substrates for the cultivation of these species. Moreover, for the effective utilisation and profitable disposal of green walnut hulls, further research is needed. to test their performance for the cultivation of other mushroom species.

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  • Atila F. 2017. Cultivation of Pleurotus spp., as an alternative solution to dispose olive waste. Journal of Agricure Ecology Research International, 12: 1–10.
  • Atila, F., Tuzel, Y., Cano, A.F. and J.A. Fernandez. 2017. Effect of different lignocellulosic wastes on Hericium americanum yield and nutritional characteristics. Journal of the Science of Food and Agriculture, 97 (2): 606–612.
  • Atila, F., Tuzel, Y., Fernandez, J.A., Cano, A.F. and F. Sen. 2018. The effect of some agro– industrial wastes on yield, nutritional characteristics and antioxidant activities of Hericium erinaceus isolates. Scientia Horticulturae, 238 (19): 246–254.
  • Balakrishnan, B. and M.C. Nair. 1995. Production technology of oyster mushroom. In: Chadha KL, Sharma SR (eds) Advances in Horticulture, Mushroom Malhotra Publishing House, New Delhi.
  • Boztok, K. and Erkip, N. 2002. Growing Shiitake Mushrooms (Lentinula edodes) On Tree Logs. Ege Üniversitesi Ziraat Fakültesi Dergisi, 39(1):149-155.
  • Celik, Y. and K. Peker. 2009. Benefit/Cost analysis of mushroom production for diversification of income in developing countries. Bulgarian Journal of Agricultural. Science, 15 (3): 228–237.
  • Curvetto, N.R., González-Matute, R., Figlas, D. and S. Delmastro. 2005. Shiitake Bag Cultivation. Chapter 4. Sunflower Seed Hulls. Mushroom Grower´s Handbook 2. Shiitake Cultivation Ed. Mushworld- Heineart Inc.
  • Chaiyasut, C. and B.S. Sivamaruthi. 2017. Anti-hyperglycemic property of Hericium erinaceus – A mini review. Asian Pacific Journal of Tropical Biomedicine, 7 (11): 1036–1040.
  • D'Abrosca, B, DellaGreca, M, Fiorentino, A, Monaco, P and A. Zarrelli. 2004. Low molecular weight phenols from the bioactive aqueous fraction of Cestrum parqui. Journal of Agriculture and Food Chemistry, 52 (13): 4101–4108.
  • Della Greca, M., Monaco, P., Pinto, G., Pollio, A., Previtera, L. and F. Temussi. 2001. Phytotoxicity of low–molecular–weight phenols from olive mill waste waters. Bulletin of Environmental Contamination and Toxicology, 67 (3): 352–359.
  • Ehlers, S. and W. Schnitzler. 2000. Studies on the growth of the basidomycete Hericium erinaceus (Bull.Ex.Fr.) Pers. Champignon. 415: 147–150.
  • Ercisli, S. and C. Turkkal. 2005. Allelopathic effects of juglone and walnut leaf extracts on growth, fruit yield and plant tissue composition in strawberry cvs. ‘Camarosa’ and ‘Sweet Charlie’. Journal of Horticultural Science and Biotechnology, 80 (1): 39–42.
  • Ercisli, S., Esitken, A., Turkkal, C. and E. Orhan. 2005. The allelopathic effects of juglone and walnut leaf extractson yield, growth, chemical and PNE compositionsof strawberry cv. Fern. Plant Soil and Environment, 51 (6): 283–287.
  • Figlas, D., González-Matute, R. and N. Curvetto. 2007. Cultivation of Hericium erinaceus on substrate containing sunflower seed hulls. International Journal of Medical Mushrooms, 9: 67–73.
  • Fukushima, M., Ohashi, T., Fujiwara, Y., Sonoyama, K. and M. Nakano. 2001. Cholesterol-lowering effects of maitake (Grifola frondosa) fiber, shiitake (Lentinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Experimental Biology And Medicine, 226 (8): 758–765.
  • Hassan, F.R.H., 2007. Cultivation of the Monkey Head Mushroom (Hericium erinaceus) in Egypt. Journal of Applied Sciences Research, 3 (10): 1229–1233.
  • Hearst, R., Nelson, D., McCollum, G., Millar, B.C., Maeda, Y., Goldsmith, C.E., Rooney, P.J., Loughrey, A., Rao, J.R. and J.E. Moore Hiraki. 2017. An examination of antibacterial and antifungal properties of constituents of Shiitake (Lentinula edodes) and Oyster (Pleurotus ostreatus) mushrooms. Complementary Therapies in Clinical Practice, 15 (1): 5–7.
  • Hiraki, E., Furuta, S., Kuwahara, R., Takemoto, N., Nagata, T., Akasaka, T., Shirouchi, B., Sato, M., Ohnuki, K. and K. Shimizu. 2017. Anti-obesity activity of Yamabushitake (Hericium erinaceus) powder in ovariectomized mice, and its potentially active compounds. Journal of Natural Medicines, 71: 482–491.
  • Kacar, B.and A. İnal. Bitki Analizleri. Nobel Yayın Dağıtım, Ankara, 2008. (In Turkish).
  • Kitzberger, C.S.G., Smania, A. Jr, Pedrosa, R.C., Regina, S. and S. Ferreira. 2007. Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids. Journal of Food Engineering, 80 (2): 631–638.
  • Ko, H.G., Park, H.G., Park, S.H., Choi, C.W., Kim, S.H. and W.M.,Park. 2005. Comparative study of mycelial growth and basidiomata formation in seven different species of the edible mushroom genus Hericium. Bioresource Technology, 96 (13): 1439–1444.
  • Kopjar, M., Tadić, M. and V. Piližota. 2015. Phenol content and antioxidant activity of green, yellow and black tea leaves. Chemical and Biological Technologies in Agriculture, 2 (1): 1–6.
  • Martínez, A.T., Speranza, M., Ruiz-Dueñas, F.J., Ferreira, P., Camarero, S., Guillén, F., Martinez, M.J., Gutierrez, A. and J.C. del Rio. 2005. Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin. International Microbiology, 8: 195–204.
  • Moonmoon, M., Shelly, N.J., Md Khan, A., Md. Uddin, N., Hossain, K., Tania, M. and S. Ahmed. 2011. Effects of different levels of wheat bran, rice bran and maize powder supplementation with sawdust on the production of shiitake mushroom (Lentinus edodes (Berk.) Singer). Saudi Journal of Biological Science 18 (4): 323–328.
  • Mori, K., Ouchi, K. and N. Hirasawa. 2015. The Anti-Inflammatory Effects of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Higher Basidiomycetes) in a Coculture System of 3T3-L1 Adipocytes and RAW264 Macrophages. International Journal of Medical Mushrooms. 17 (7): 609–618.
  • Naheed, A and S. Iqbal-Zafar. 1999. Lignocellulose biodegradation by white rot basidiomycetes: overview. International Journal of Mushroom Science, 2: 59–78.
  • Nicolini, L., Von Hunolstein, C. and A. Carilli. 1987. Solid state fermentation of orange peel and grape stalks by Pleurotus ostreatus, Agrocybe aegerita a nd Armillaria mellea. Applied Microbiology and Biotechnology, 26: 95–98.
  • Ozcelik, E. and A. Peksen. 2007. Hazelnut husk as a substrate for the cultivation of shiitake mushroom (Lentinula edodes). Bioresource Technology, 98 (14): 2652–2658.
  • Onyango, B.O., Palapala, V.A., Arama, P.F., Wagai, S.O. and B.M. Gichumu. 2011. Sustainability of selected supplemented substrates for cultivation of Kenyan native wood ear mushrooms (Auricularia auricula). American Journal of Food Technology, 6 (5): 395–403.
  • Pardo A, Perona MA and J. Pardo. 2007. Indoor composting of vine by-products to produce substrates for mushroom cultivation. Spanish Journal of Agricultural Research, 5 (3): 417–424.
  • Philippoussis, A., Zervakis, G. and P. Diamantopoulou. 2001. Bioconversion of agricultural lignocellulosic wastes through the cultivation of the edible mushrooms Agrocybe aegenita, Volvariella volvacea and Pleurotus spp. World Journal of Microbiology and Biotechnology, 17 (2): 191–200.
  • Philippoussis, A., Diamantopoulou, P. and G. Zervakis. 2003. Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinus edodes. World Journal of Microbiology and Biotechnology, 19 (6): 551–557.
  • Philippoussis, A., Diamantopoulou, P. and C., Israilides. 2007. Productivity of agricultural residues used for the cultivation of the medicinal fungus Lentinula edodes. International Biodeterioration & Biodegradation, 59 (3): 216–219.
  • Ramirez-Lopez, L.M. and C.A.M. DeWitt. 2014. Analysis of phenolic compounds in commercial dried grape pomace by high-performance liquid chromatography electrospray ionization mass spectrometry. Food Science and Nutrition, 2 (5): 470–477.
  • Royse DJ and B.D. Bahler. 1986 Effects of genotype, spawn run time, and substrate formulation on biological efficiency of shiitake. Applied Environmental Microbiology, 52: 1425–1427.
  • Royse, D.J., 1997. Speciality mushrooms and their cultivation. Horticultural Reviews, 19: 59–97.
  • Royse, D.J., Rhodes, T.W., Ohga, S. and J.E. Sánchez. 2004. Yield, mushroom size and time to production of Pleurotus cornucopiae (oyster mushroom) grown on switch grass substrate spawned and supplemented at various rates. Bioresource Technology, 91 (1): 85–91.
  • Royse, D. and J.E. Sanchez. 2007. Ground wheat straw as a substitute for portions of oak wood chips used in shiitake (Lentinula edodes) substrate formulae. Bioresource Technology, 98 (11): 2137–2141.
  • Ruiz-Rodriguez, A., Soler-Rivas, C., Polonia, I. and J.H. Wichers. 2010. Effect of olive mill waste (OMW) supplementation to oyster mushrooms substrates on the cultivation parameters and fruiting bodies quality. International Biodeterioration & Biodegradation, 64 (7): 638–645.
  • Siwulski, M., Sobieralski, K. and M. Wojnilowicz. 2005. Influence of some growing substrate additives on the Hericium erinaceus (Bull. Fr.) Pers. yield. Sodininkystė ir Daržininkystė. 24 (3): 250–253.
  • Spelman, K., Sutherland, E. and A. Bagade. 2017. Neurological Activity of Lion’s Mane (Hericium erinaceus). Journal of Restorative Medicine, 6: 19–26.
  • Stamets, P. 1993. Growing Gourment and Medicinal Mushrooms. Ten Speed Press and Mycomedia, Berekely
  • Stampar, F., Solar, A., Hudina, M., Veberic, R. and M. Colaric. 2006. Traditional walnut liqueur – cocktail of phenolics. Food Chemistry, 95 (4): 627–631.
  • Terzi, I. 2008. Allelopathic effects of Juglone and decomposed walnut leaf juice on muskmelon and cucumber seed germination and seedling growth. African Journal of Biotechnology, 7 (12): 1870–1874.
  • Vlyssides, A.G., Loizides, M. and P.K. Karlis. 2004. Integrated strategic approach for reusing olive oil extraction. Journal of Cleaner Prodoction, 12 (6): 603–611.
  • Worralli, J.J. and C.S. Yang. 1992. Shiitake and oyster mushroom production on apple pomace and sawdust. HortScience 27 (10): 1131–1133.
  • Yang D, Liang J, Wang Y, Sun F, Tao H, Xu Q, Zhang L, Zhang Z, Ho CT and X. Wan. 2016. Tea waste: An effective and economic substrate for oyster mushroom cultivation. Journal of the Science of Food and Agriculture, 96 (2): 680–684
  • Zervakis, G.I., Koutrotsios, G. and P. Katsaris. 2013. Composted versus raw olive mill waste as substrates for the production of medicinal mushrooms: An assessment of selected cultivation and quality parameters. Biomed. Res. Int. Article ID 546830, http://dx.doi.org/10.1155/2013/546830.
  • Zhang, H., Gao, J.M., Liu, W.T., Tang, J.C., Zhang, X.C., Jin, Z.G., Xu, Y.P. and M.A. Shao, 2008. Allelopathic substances from walnut (Juglans regia L) leaves. Allelopathy Journal, 21 (2): 354–362.
  • Zhang, Y., Ming, G., Kaiping, W., Zhixiang, C., Liquan, D., Jingyu, L. and Z. Fang. 2010. Structure, chain conformation and antitumor activity of a novel polysaccharide from Lentinus edodes. Fitoterapia 81 (8): 1163–1170.
  • Zied, D.C., Pardo, J.E., Pardo, A. and Mta., Minhoni. 2009. Cultivo de Shiitake, Lentinula edodes (Berk.) Pegler, en substrato formulado. In: Jornadas, V. ed., Consejería de Agricultura y Medio Ambiente y Diputación Provincial de Cuenca, Técnicas del Champiñón y otros Hongos Comestibles en Castilla-La Mancha