Mostafa NORİZADEH TAZEHKAND, Mehmet Bertan YILMAZ

Klorzoksazonun antibakteriyel etkisinin belirlenmesi

Amaç: Antibiyotik olmayan ilaçların bazıları, rutin kullanımlarında bakteri gelişimine karşı inhibisyon etki sağlayabilirler. Klorzoksazon, kas spazmı ve bununla birlikte ortaya çıkan ağrıyı tedavi etmek için kullanılan kas gevşetici ilaçtır. Bu çalışmanın amacı Klorzoksazon ‘un antibakteriyel etkilerinin MİK (minimum inhibitör konsantrasyon), MBK (minimum bakterisid konsantrasyon) ve disk difüzyon metodu ile incelenmesidir.Gereç ve Yöntem: Klorzoksazon’a karşı MİK ve MBK değerlerin belirlenmesi 2 farklı bakteri suşunda (gram negatif bakteri suşu olarak Pseudomonas aeruginosa ve gram pozitif bakteri suşu olarak Bacillus subtilis) seyreltmesi yöntemine göre belirlenmiştir. Disk difüzyon testi ise üç farklı konsantrasyon (25, 50 ve 100 μg)’da yapılmıştır. Bulgular: Klorzoksazon’un Pseudomonas aeruginosa'ya karşı MİK değeri 800 mg mL-1, MBK değerleri ise 2.56 mg mL-1 ve Klorzoksazon 'un Bacillus subtilis'e karşı MİK değerleri 0.400 mg mL-1, MBK değeri ise 1.92 mg mL-1 olarak belirlenmiştir. Disk difüzyon deneyinden elde edilen sonuçlar Klorzoksazon’un Pseudomonas aeruginosa ve Bacillus subtilis üzerinde antibakteriyel etkisinin olmadığı sonuçlanmıştır. Sonuç: Klorzoksazon’un antibakteriyel aktivitesinin olmamasına rağmen Klorzoksazon’un muhtemel riskini belirlemek için başka çalışmalara ihtiyaç vardır.

Anahtar Kelimeler:

Antibakteriyel etki, Bacillus subtilis, Klorzoksazon, Pseudomonas aeruginosa

Evaluation of antibacterial effects of chlorzoxazone

Purpose: Some of non-antibiotic drugs may provide inhibition against bacterial growth in their routine use. Chlorzoxazone is a muscle relaxant used to treat muscle spasm and the resulting pain or discomfort. This study was aimed to the investigation of antibacterial effects of Chlorzoxazone using MIC (Minimum inhibitory concentration), MBC (minimum bactericidal concentration) and disk diffusion method. Materials and Methods: The MIC and MBC was determined by dilution assay using different concentrations of Chlorzoxazone against 2 bacterial strain (Pseudomonas aeruginosa as a gram negative bacterial strain and Bacillus subtilis as a gram positive bacterial strain). Disk diffusion assay were prepared at different doses (25, 50 and 100 µg). Results: MIC values of Chlorzoxazone against Pseudomonas aeruginosa was 0.800 mg ml-1, and MBC values was 2.56 mg ml-1 and MIC values of Chlorzoxazone against Bacillus subtilis was 0.400 mg ml-1, and MBC values was 1.92 mg ml-1. The results obtained from disk diffusion assay supported that the Chlorzoxazone has not antibacterial effect on Pseudomonas aeruginosa and Bacillus subtilis. Conclusion: Although antibacterial activity of Chlorzoxazone was not found but another studies are needed to determine the probable risk of Chlorzoxazone.

Keywords:

Antibacterial effect, Bacillus subtilis, chlorzoxazone,

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