ROKA (ERUCA SATİVA) BİTKİSİNİN KÖK VE GÖVDESİNDEKİ ANTİOKSİDAN ENZİM AKTİVİTELERİ, METAL BİRİKİMİ, BAZI FİZYOLOJİK VE BÜYÜME PARAMETRELERİNDEKİ DEĞİŞİMLERİN ARAŞTIRILMASI - v

Öz ROKA (ERUCA SATİVA) BİTKİSİNİN KÖK VE GÖVDESİNDEKİ ANTİOKSİDAN ENZİM AKTİVİTELERİ, METAL BİRİKİMİ, BAZI FİZYOLOJİK VE BÜYÜME PARAMETRELERİNDEKİ DEĞİŞİMLERİN ARAŞTIRILMASIBu çalışmada, farklı Mn+2 (10 - 5000 μM) veya Fe+2 (50 – 5000 μM) konsantrasyonlarının varlığındaEruca sativa bitkisindeki büyüme geriliği ile ilişkili olarak köklerde ve gövdede antioksidatif cevabınkarşılaştırılmasını amaçladık. Aşırı Mn+2 veya Fe+2 bitkilerin büyümesini anlamlı olarak inhibe etti. Her ikidokuda da, Mn+2 ve Fe+2 stresinde MDA, prolin ve H2O2 seviyelerinde, elektrolitik iletkenlik, SOD ve GPXaktivitelerinde anlamlı artış izlendi. Eruca sativa bitkisinde, dışarıdan artan konsantrasyonlarda uygulananMn+2 ve Fe+2, bitkinin köklerinde ve gövdesinde uygulanan metalin aşırı birikimine sebep oldu. Aşırı Mn+2köklerde ve gövdede Fe+2 ve Zn+2 alınımını anlamlı olarak azaltırken Cu içeriğini arttırdı. Sonuç olarak,yüksek Mn+2 veya Fe+2 içeriği oksidatif stresi arttırarak büyümenin baskılanmasından sorumlu tutulabilir.STUDY OF THE CHANGE IN ANTIOXIDATIVE ENZYME ACTIVITIES, METAL ACCUMULATION, SOME PHYSIOLOGICAL AND GROWTH PARAMETERS OF SHOOT AND ROOT IN ROCA (ERUCA SATIVA) PLANTIn this study, we aimed to compare some antioxidative responses, which is associated with thegrowth reduction, of shoots and roots of Eruca sativa plant in the presence of different concentration of Mn+2(10 - 5000 μM) or Fe+2 (50 – 5000 μM). The excess of Mn+2 or Fe+2 caused a significant inhibition on theplant growth. Significant increases in MDA, SOD, GPX, proline, electrolytic leakage and H2O2 levels wereobserved in both shoots and roots stressed under Mn+2 and Fe+2. Mn+2 treatment caused a greater decreasein growth than Fe+2 treatment in both tissues. The contents of total chlorophyll and carotenoid in the leaves ofthe plant were also reduced by the increasing the Mn+2 or Fe+2 concentrations. Cu uptake was increased in thepresence of excess Mn+2 in both tissues, although Fe+2 and Zn+2 contents were significantly reduced.Consequently, it can be concluded, that the high Mn+2 or Fe+2 contents in growth media can be theresponsible for the growth inhibition by enhancing the oxidative stress.

Anahtar Kelimeler:

Roka (Eruca sativa) bitkisi, Mn+2 ve Fe+2 stresi, metal alımı, lipid peroksidasyonu

Roka (Eruca Sativa) Bitkisinin Kök ve Gövdesindeki Antioksidan Enzim Aktiviteleri, Metal Birikimi, Bazı Fizyolojik ve Büyüme Parametrelerindeki Değişimlerin Araştırılması

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Kabul Tarihi:28/09/2010