FARE AKCİGER GELİSİMİNDE GLUKOKORTİKOİDLERİN FİBRONEKTİN, KADERİN VE BETA-KATENİN MOLEKÜLLERİNİN DAGILISLARI ÜZERİNE ETKİSİ - THE EFFECTS OF GLUCOCORTICOIDS ON THE DISTRIBUTION OF FIBRONECTIN, CADHERIN AND BETA-CATENIN MOLECULES DURING MOUSE LUNG DEVELOPM

Öz FARE AKCİGER GELİSİMİNDE GLUKOKORTİKOİDLERİN FİBRONEKTİN, KADERİN VE BETA-KATENİN MOLEKÜLLERİNİN DAGILISLARI ÜZERİNE ETKİSİMemeli akciger gelisimi hücreler ile ekstrasellüler matriksler arasında gerçeklesen kompleksetkilesimleri içerir. Bu süreç, fibronektin, laminin, kaderin, katenin ve kollajen gibi bir dizi matriks bilesenive adezyon molekülü tarafından düzenlenmektedir. Glukokortikoidler fötal akciger gelisiminde merkezi birrole sahiptir. Örnegin, gebelik sırasında glukokortikoid uygulamasının akciger gelisiminde degisiklige nedenoldugu bilinmektedir. Bu çalısmada, eksojen glukokortikoidlerin fibronektin, kaderin ve beta-kateninmoleküllerinin gelisimsel profili üzerine etkilerini belirlemek için immunohistokimyasal bir yöntemuygulandı. Deksametazon gebeligin 11., 12., 13. ve 14. günlerinde hamile farelere verildi. Hayvanlar 12., 13.,14. ve 15. günlerde kesildi ve fibronektin, pan-kaderin ve beta-katenin poliklonal antikorları ile boyandı.Bulgularımız, distal epitelyum, iletici hava kanalları, bazal membranlar, kanalların lümene bakan yüzeylerive mezensim hücrelerinde fibronektin, pan-kaderin ve beta-katenin reaktivitelerinin benzer oldugunugösterdi. Bu sonuçlar, 24 saatlik dogum öncesi deksametazon uygulamalarının incelenen devrelerde gelisenakciger dokularında fibronektin, kaderin ve beta-katenin içerikleri üzerine tam bir etkisinin olmadıgınıdüsündürmüstür.THE EFFECTS OF GLUCOCORTICOIDS ON THE DISTRIBUTION OF FIBRONECTIN, CADHERIN AND BETA-CATENIN MOLECULES DURING MOUSE LUNG DEVELOPMENTMammalian lung development involves complex relationships between cells and extracellularmatrices. This process mediated by a series of matrix components and adhesion molecules such asfibronectin, laminin, cadherin, catenin and collagen. Glucocorticoids play a central role in fetal lungdevelopment. For example it is known that, glucocorticoid administration during pregnancy alters thematuration of the lung. In the present study, we used an immunohistochemical method to evaluate thepossible effect of exogenous glucocorticoids to developmental profile of fibronectin, cadherin and betacateninmolecules. Dexamethasone was administrated to pregnant mice on days 11, 12, 13 and 14 ofgestation. Animals were sacrificed on days 12, 13, 14 and 15, and stained with polyclonal antibodies tofibronectin, pan-cadherin and beta-catenin. Our results indicated that staining reactivities of fibronectin, pancadherinand beta-catenin were similar in distal epithelia, conductive airways, basal membranes, luminalsurfaces of airways and mesenchymal cells. Therefore, we suggest that, 24 hourly antenatal dexamethasonetreatments couldn’t completely affect fibronectin, cadherin and beta-catenin contents in evaluated periods ofdeveloping lung.

Fare Akçiğer Gelişiminde Glukokortikoşdlerin Fibronektin, Kaderin ve Beta-Katenin Moleküllerinin Dağılışları Üzerine Etkisi

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Geliş Tarihi: 20/01/2009 Kabul Tarihi: 09/04/2009

Celal Bayar Üniversitesi Fen Bilimleri Dergisi-Cover
  • ISSN: 1305-130X
  • Başlangıç: 2005
  • Yayıncı: Manisa Celal Bayar Üniversitesi Fen Bilimleri Enstitüsü
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