Effective Reduction of Graphene Oxide via Lactococcus lactis

In this study, bacteria Lactococcus lactis was employed as a bioreducing agent for ecofriendly and economical production of reduced graphene oxide at room temperature. Characterizations have showed that highly reduced graphene oxide was produced in mild conditions and layers have low number of wrinkles. Exfoliation by Lactococcus lactis has been succeeded to produce single or few layer reduced graphene oxide. Decrease in ratio of ID/IG from 2.15 to 0.97 calculated from Raman spectrum, decrease and/or disappearance of characteristic peaks of oxygen functional groups from FTIR, and changes observed in 2theta characteristic peak values at XRD spectrum have confirmed the reduction success of Lactococcus lactis. These results have indicated that Lactococcus lactis biomass could be employed as a new reductant to prevent the use of harmful chemicals and harsh conditions for reduced graphene oxide generation having high stability.

___

  • [1]. Geim, AK, Grigorieva, IV. 2013. Van der Waals heterostructures. Nature; 499: 419-425.
  • [2]. Hummers, Jr. WS, Offeman, RE., 1958. Preparation of Graphitic Oxide. Journal of American Chemical Society; 80(6): 1339–1339.
  • [3]. Aunkor, MTH, Mahbubul, IM, Saidur, R, Metselaar, HSC. 2016. The green reduction of graphene oxide. The Royal Society of Chemistry Advances; 6(33): 27807– 27828.
  • [4]. Gurunathan, S, Han, JW, Eppakayala, V, Kim, JH. 2013. Microbial reduction of graphene oxide by Escherichia coli: a green chemistry approach, Colloids and Surfaces B: Biointerfaces; 102: 772- 777.
  • [5]. Salas, EC, Sun, Z, Lüttge, A, Tour, JM. 2010. Reduction of graphene oxide via bacterial respiration. American Chemical Society Nano: 4(8): 4852– 48566.
  • [6]. Wang, G, Qian, F, Saltikov, CW, Jiao, Y, Li, Y. 2011. Microbial reduction of graphene oxide by Shewanella. Nano Research; 4(6): 563– 570.
  • [7]. Zhang, H, Yu, X, Guo, D, Qu, B, Zhang, M, Li, Q, Wang, T. 2013. Synthesis of bacteria promoted reduced graphene oxide-nickel sulfide networks for advanced supercapacitors. American Chemical Society Applied Materials Interfaces; 5: 7335– 7340.
  • [8]. Raveendran, S, Chauhan, N, Nakajima, Y, Toshiaki, H, Kurosu, S, Tanizawa, Y, Tero, R, Yoshida, Y, Hanajiri, T, Maekawa, T, Ajayan, PM, Sandhu, A, Kumar, DS. 2013. Ecofriendly route for the synthesis of highly conductive graphene using extremophiles for green electronics and bioscience. Particles &. Particle Systems Characterizations; 30: 573– 578.
  • [9]. Chen, Y, Niu, Y, Tian, T, Zhang, J, Wang, Y, Li, Y Qin, LC. 2017. Microbial reduction of graphene oxide by Azotobacter chroococcum. Chemical Physics Letters; 677: 143–147.
  • [10]. Lehner, BAE, Janssen, VAEC, Spiesz, EM, Benz, D, Brouns, SJJ, Meyer, AS, van der Zant, HSJ. 2019. Creation of Conductive Graphene Materials by Bacterial Reduction Using Shewanella Oneidensis. ChemistryOpen; 8: 888–895.
  • [11]. Fan, M, Zhu, C, Feng, ZQ, Yang, J, Liu, L, Sun, D. 2014. Preparation of N-doped graphene by reduction of graphene oxide with mixed microbial system and its haemocompatibility. Nanoscale; 6: 4882- 4888.
  • [12]. Priyadarshani Choudhary, P., Das, SK. 2019. Bio-Reduced Graphene Oxide as a Nanoscale Antimicrobial Coating for Medical Devices. ACS Omega; 4: 387−397.
  • [13]. Gurunathan, S, Han, JW, Eppakayala, V., Kim, JH. 2013. Green synthesis of graphene and its cytotoxic effects in human breast cancer cells. Int. J. Nanomedicine; 8: 1015–1027.
  • [14]. Schütz, B, Seidel, J, Sturm, G, Einsle, O, Gescher, J. 2011. Investigation of the electron transport chain to and the catalytic activity of the diheme cytochrome c peroxidase CcpA of Shewanella oneidensis. Applied Environmental Microbiology; 77: 6172– 6180.
  • [15]. Utkan, G, Ozturk T, Duygulu O, Tahtasakal, E, Denizci, AA. 2019. Microbial Reduction of Graphene Oxide by Lactobacillus plantarum. International Journal of Nanoscience and Nanotechnology; 15(2): 127-136 (24).
  • [16]. Zhou, X, Zhang, J, Wu, H, Yang, H, Zhang, J, Guo, S. 2011. Reducing graphene oxide via hydroxylamine: a simple and efficient route to graphene. J. Phys. Chem. C; 115(24): 11957–11961.
  • [17]. Zhang, J, Yang, H, Shen, G, Cheng, P, Zhang J, Guo, S. 2010. Reduction of graphene oxide via L-ascorbic acid. Chem. Commun.; 46(7): 1112–1114.
  • [18]. Wang, Y, Shi, Z, Yin, J. 2011. Facile synthesis of soluble graphene via a green reduction of graphene oxide in tea solution and its biocomposites, ACS Appl. Mater. Interfaces; 3(4):1127–1133.
  • [19]. Khanra, P, Kuila, T, Kim, NH, Bae, SH, Yu, DS, Lee, JH. 2012. Simultaneous bio-functionalization and reduction of graphene oxide by baker's yeast, Chem. Eng. J.; 183, :526–533.
  • [20]. Aunkor, M, Mahbubul, I, Saidur, R, Metselaar, HSC. 2015. Deoxygenation of graphene oxide using household baking soda as a reducing agent: a green approach. RSC Adv.; 5(86): 70461–70472.
  • [21]. Akhavan, O, Ghaderi, E. 2012. Escherichia coli bacteria reduce graphene oxide to bactericidal graphene in a self-limiting manner. Carbon; 50: 1853– 1860.
  • [22]. Fernandez-Merino, M, Guardia, L, Paredes, J, VillarRodil, S, Solis-Fernandez, P, Martinez-Alonso, A, Tascon, JMD. 2010. Vitamin C is an ideal substitute for hydrazine in the reduction of graphene oxide suspensions, J. Phys. Chem. C; 114(14): 6426–6432.
  • [23]. Lei, Y, Tang, Z, Liao, R, Guo, B. 2011. Hydrolysable tannin as environmentally friendly reducer and stabilizer for graphene oxide. Green Chem.; 13(7); 1655–1658.
  • [24]. Fu, C, Zhao, G, Zhang, H, Li, S. 2013. Evaluation and Characterization of Reduced Graphene Oxide Nanosheets as Anode Materials for Lithium-Ion Batteries. Int. J. Electrochem. Sci; 8: 6269 – 6280.
  • [25]. Aliyev, E, Filiz, V, Khan,, MM, Lee, YJ, Abetz, C, Abetz, V. 2019. Structural Characterization of Graphene Oxide: Surface Functional Groups and Fractionated Oxidative Debris. Nanomaterials; 9: 1180-1195.
  • [26]. Azizighannad, S, Mitra, S. 2018. Stepwise Reduction of Graphen Oxide (GO) and Its Effects on Chemical and Colloidal Properties. Nature Scientific Reports; 8: 10083.
  • [27]. Gurunathan, S, Han, Kim, JH. 2013. Green chemistry approach for the synthesis of biocompatible graphene. Int. J. Nanomedicine; 8: 2719-2732.
  • [28]. Ghosh, TK, Gope, S, Rana, D, Roy, I, Sarkar, G Sadhukhan, S, Bhattacharya, A, Pramanik, K, Chattopadhy, S, Chakraborty, M, Chattopadhyay, D. 2016. Physical and electrical characterization of reduced graphene oxide synthesized adopting green route. Bull. Mater. Sci.; 39 (2): 543–550.
  • [29]. Jiang RR, Riebel BR, Bommarius AS. 2005. Comparison of alkyl hydroperoxide reductase (AhpR) and water-forming NADH oxidase from Lactococcus lactis ATCC 19435. Advanced Synthesis & Catalysis; 347: 1139-1146.
  • [30]. van Niel EWJ, Hofvendahl K, Hahn-Hagerdal B. 2002. Formation and conversion of oxygen metabolites by Lactococcus lactis subsp lactis ATCC 19435 under different growth conditions. Applied and Environmental Microbiology; 68: 4350-4356.