Cytospora chrysosperma "Pers" Fr. İle In-Vitro Koşullarında Morfolojik Özelliklerinin ve En Uygun İnokulasyon Yönteminin Belirlenmesi

Cytospora chrysosperma “Pers” Fr.’nın PDA (Patates dekstroz-Agar) besi ortamında 24 ±1ºC’de ve %80 orantılı

Anahtar Kelimeler:

İn-vitro, Cytospora chrysosperma, Kavak, Patojenisite

Determination of Morphological Features and the Best Inocculation Method of Cytospora chrysosperma “Pers” Fr. at In-vitro Conditions

It was determined that Cytospora chrysosperma “Pers” Fr. developed best at PDA (Potato Dextrose Agar)nourishment media and at 24±1°C and 80% relative humidity conditions. Development rate of the pathogen wasfound to be 14 mm/day and under these conditions, pycnidia production occurred 3 days after incubation andspores secreted from these pycnidia after 24 days after incubation. It was also determined that the disease agentproduces multioccular form pycnidia on infected poplar barks and yellowish-pink or dark red coloreddeterministic spores were secreted from these pycnidia. The disease agent may produce various virulenceisolates depending on climatic conditions. It was determined that the isolate 25 (Ankara-Kırıkkale isolate) wasthe most virulent isolate in the study area. It was once more determined that the disease agent to be a pathogen ofpoplars and willows were found to be hosts of the disease.

Keywords:

In-vitro, Cytospora chrysosperma, Poplar, Pathogenicity,

PDF

___

o Agrios 1997. Plant Pathology. Fourth Edition, Academic press. USA, 634p.
o Aktaş, H., Şimşek, Z. 2005. Çankırı Kenbağ Orman Fidanlığındaki Kavak Fidanlarında Cytospora Kanseri (Cytospora chrysosperma “Pers.” Fr.)’Nin Morfolojisi, Zararı Ve Alınabilcek Önlemler, İstanbul Üniversitesi Orman Fakültesi Dergisi Seri A, Cilt 55, Sayı 2, 47-57.
o Aktaş, H., Şimşek, Z., Kondur, Y. 2008. Türkiye'de Orta Anadolu Bölgesi'nde Kavak Kanseri (Cytospora chrysosperma "Pers." Fr.)'nin Morfolojik Özellikleri, Zarar Durumu ve Sarı Lekeli Kavak Süslüböceği (Melanophila picta Pall. (Coleoptera: Buprestidae) Arasındaki İlişkiler. Bitki Koruma Bülteni 48(3): 1-14.
o Anonymous 2002. www.pfc.forestry.ca/diseases/CTD/Group/Canker/canker2_e.html
o Butin, H. 1995. Tree Diseases and Disorders. Oxford University Pres, 252p.
o Domanski, S. 1983. Fungi That Destroyed A Populus Tremula Stand in Lagow Lubuski. European Journal of Forest Pathology, 166-173.
o Filler, T., Randall, W.K. 1991. Variation Among Cottonwood Clones in Susceptibility to Cytospora, Phomopsis and Fusarium. Proceedings Of The American Phytopathological Society, 4: 83p.
o Jacobi, W.R., Shepperd, W.D. 1991. Fungi Associated With Sprout Mortality İn Apsen Clearcuts in Colaroda And Arizona. Usda Forest Service, No. Rm. 513, 5p.
o Karman, M. 1971. Bitki Koruma Araştırmalarında Genel Bilgiler, Denemelerin Kuruluşu ve Değerlendirme Esasları. T.C. Tarım Bak. Zir. Müc. Ve Karantina Gn. Md. Yayınları, Mesleki Kitaplar Serisi, 279 S.
o Keca, N. 2000. The Most Frequent Diseases in Poplar Plantations in The Region of R.J. Potisje. Glasnik Sumarskog Fakulteta. Univerzitet U Beogradu., No.82, 81-91.
o Kepley, J.B., Jacobi, W.R. 2000. Pathogenicity of Cytospora Fungi on Six Hardwood Species. Journal of Arboriculture., 26: 6, 326-333.
o Koçer, S. 1999. Ülkemizde Kavakçılığın Geliştirilmesinde Yeni Finansman Olanakları, Orman Bakanlığı Kavak ve Hızlı Gelişen Ağaçlar Araştırma Enstitüsü Teknik Bülten No:170, İzmit.
o Long, W.H. 1918. An Undescribed Canker of Poplars and Willows Caused by Cytospora chrysosperma. Journal of Agricultural Research, 13:331-343.
o Mader, Z, Solel, Z., Kimchi, M. 2004. First Report of Cytospora Canker caused by Cytospora chrysosperma on white poplar in İsrael. Plant Dis., 88: 220
o Peace, T.R. 1962. Parthology of Trees and Shrubs, Oxfort Univ. Press, London, 753p.
o Uluer, K., Gürer, M., Güler, N. 1998. Kavaklarda C. chrysosperma (Pers.) Fr. Zararını Önleme Üzerine Araştırmalar. Orm. Bak. Yayını, Issn 1300 - 395 X. İzmit, (1998/3), 25 s.