Yıl 2017, Cilt: 47 Sayı : 1 Sayfalar 5 - 8 2017-10-10
Cytotoxicity and genotoxicity of fenoxaprop-p-ethyl and fluzifob-p-butyl herbicides
15 261

Öz Human populations are exposed to several toxic substances in the environment, including pesticides. Fenoxaprop-p-ethyl (FPE) and fluzifob-p-butyl (FPB) herbicides are extensively used in agricultural fields due to their high target selectivity and low non-target toxicity. They are known as aryloxyphenoxypropionate herbicides, and acetyl-CoA carboxylase inhibitor. In the study, we aimed to evaluate the toxic potentials of FPE and FPB herbicides. Cell viability was evaluated by MTT assay in the range of 15.6-500.0 μM exposure concentrations in mouse fibroblast (BALB/3T3) cell line. Also, we investigated their DNA damage potentials on BALB/3T3 cells by using alkaline Comet assay. The results indicated that FPE and FPB showed no evidence of DNA damage. And, the cell viability was more than 20% at 12.5-400 μM exposure concentrations. FPE and FPB might be safe according to our results and the previously studies, and there would be public health benefits from encouraging its use in the place of more toxic herbicide products.

Anahtar Kelimeler

Fenoxaprop-p-ethyl, fluzifob-p-butyl, 3T3 cells
Abd-Alrahman SH, Elhalwagy ME, Kotb GA, Farid H, FaragAA, Draz HM, Isa AM, Sabico S (2014). Exposure to difenoconazole,diclofop-methyl alone and combination altersoxidative stress and biochemical parameters in albinorats. Int J Clin Exp Med 7 (10): 3637.• Abudayyak M, Altıncekic T, Özhan G (2017). In vitro toxicologicalassessment of cobalt ferrite nanoparticles in severalmammalian cell types. Biol Trace Element Res 175 (2):458-465. [CrossRef ]• Asshauer J, Dorn E, Fischer R, Kurzmeier E (1990). Behaviourof fenoxaprop-ethyl in the environment-degradationand effect in the ecosystem. Z Pflanzenkr Pflanzenschutz12: 417-425.• Bakkali Y, Ruiz-Santaella J P, Osuna M D, Wagner J, FischerA J, De Prado R (2007). Late watergrass (Echinochloaphyllopogon): mechanisms involved in the resistance tofenoxaprop-p-ethyl. J Agric Food Chem 55 (10): 4052-4058.[CrossRef ]• Betancourt M, Reséndiz A (2006). Effect of two insecticidesand two herbicides on the porcine sperm motilitypatterns using computer-assisted semen analysis (CASA)in vitro. Reprod Toxicol 22 (3): 508-512. [CrossRef ]• Bieringer H, Horlein G, Langeluddeke P, Handte R (1982).HOE 33171: A new selective herbicide for the control ofannual and perennial warm climate grass weeds in broadleafcrops, In. Proc Brighton Crop Prot Conf-Weeds 1: 11-17.• Casas E, Bonilla E, Ducolomb Y, Betancourt M (2010). Differentialeffects of herbicides atrazine and fenoxapropethyl,and insecticides diazinon and malathion, on viabilityand maturation of porcine oocytes in vitro. Toxicol inVitro 24 (1): 224-230. [CrossRef ]• Dorn E, Schmidt E, Rutz U, Kellner HM, Leist KH (1985). Metabolismin male and female rats after single and repeatedoral administration, respectively of a low and a high dose.Hoechst-Roussel Agri-Vet Company, DPR, Pesticide RegistrationLibrary Doc. No. 51910-519031.• Extoxnet (1996). Fluazifop-p-butyl. Pesticide informationprofiles. Extension Toxicology Network. http://ace.orst.edu/info/extoxnet/• Hay A (1981). Herbicide safety: Bill of health. Nature 289:4-5. [CrossRef ]• Hongming L, Xu L, Zhaojian G, Fan Y, Dingbin C, JianchunZ, Jianhong X, Shunpeng L, Qing H (2015). Isolation of anaryloxyphenoxy propanoate (AOPP) herbicide-degradingstrain Rhodococcus ruber JPL-2 and the cloning of a novelcarboxylesterase gene (feh). Braz J Microbiol 46 (2): 425-432. [CrossRef ]• Horbowicz M, Sempruch C, Kosson R, Koczkodaj D, WalasD (2013). Effect of fluazifop-p-butyl treatment onpigments and polyamines level within tissues of nontargetmaize plants. Pestic Biochem Physiol 107 (1): 78-85. [CrossRef ]• Karadeniz A, Kaya B, Savaş B, Topcuoğlu F (2015). Effects oftwo herbicides, fluazyfop-p-butyl and fenoxaprop-p-ethyl,on genotoxicity in drosophila smart assay and on proliferationand viability of hek293 cells from the perspectiveof carcinogenesis. Fresen Environ Bull 24 (6): 2052-2054.• Kostka G, Palut D, Ludwicki JK, Kopeć-Szlęzak J, WiadrowskaB, Lembowicz K (2002). Hepatocellular peroxisomeproliferation and DNA synthesis in Wistar rats treated withherbicide fluazifop. Toxicol 178 (3): 221-228. [CrossRef ]• Lin J, Chen J, Cai X, Qiao X, Huang L, Wang D, Wang Z (2007).Evolution of toxicity upon hydrolysis of fenoxaprop-p-ethyl.J Agric Food Chem 55 (18): 7626-7629. [CrossRef ]• Morrison HI, Wilkins K, Semenciw R, Mao Y, Wigle D (1992).Herbicides and cancer. J Natl Cancer Inst 84: 1866-1874.[CrossRef ]• Olszyk D, Blakeley‐Smith M, Pfleeger T, Lee E H, Plocher M(2013). Effects of low levels of herbicides on prairie speciesof the Willamette Valley, Oregon. Environ Toxicol Chem 32(11): 2542-2551. [CrossRef ]• Ore A, Olayinka ET (2016). Fluazifop-p-butyl, an aryloxyphenoxypropionateherbicide, diminishes renal and hepaticfunctions and triggers testicular oxidative stress inorally exposed rats. Toxicol Ind. Health 0748233716657763.• Peterson DE, Regehr DL, Thompson CR, Al-Khatib K,(2011). Herbicide Mode of Action. Kansas State University,Kansas, USA, 9-15.• Saunders DS, Harper C (1994). Pesticides. In: Hayes AW(ed.), Principles and Methods of Toxicology, 3rd ed. RavenPress, New York, pp. 389-415.• Shimabukuro R H, Hoffer B L (1995). Enantiomers of diclofop-methyl and their role in herbicide mechanism of action.Pestic Biochem Physiol 51 (1): 68-82. [CrossRef ]• Speit G, Hartmann A (1999). The comet assay (Single-CellGel Test) a sensitive genotoxicity test for the detection ofDNA damage and repair. DNA Repair Protocols: EukaryoticSystems, 203-212. [CrossRef ]• Tehranchian P, Norsworthy J.K, Korres NE, McElroy S, ChenS, Scott, RC (2016). Resistance to aryloxyphenoxypropionateherbicides in Amazon sprangletop: Confirmation,control, and molecular basis of resistance. Pestic BiochemPhysiol 133: 79-84. [CrossRef ]• Tong L (2005). Acetyl-coenzyme a carboxylase: crucialmetabolic enzyme and attractive target for drug discovery.Cell Mol Biol 62 (16): 1784-1803. [CrossRef ]• Tu M, Hurd C, Randall JM (2001). Weed control methodshandbook: tools & techniques for use in natural areas. TheNature Conservancy, Report.• Van Meerloo J, Kaspers GJ, Cloos J (2011). Cell sensitivityassays: the MTT assay. Cancer cell culture: methods and protocols,237-245. [CrossRef ]• Walia U, Brar L, Dhaliwal B (1998). Performance of clodinafopand fenoxaprop-p-ethyl for the control of phalarisminor in wheat. Ind J Weed Sci 30: 48-50.• Yasin M, Iqbal Z, Safdar ME, Rehman A, Ali A, Asif M, AzizM, Tanveer A, Pervez MA (2011). Phalaris minor control, resistancedevelopment and strategies for integrated managementof resistance to fenoxaprop-ethyl. Afr J Biotechnol10 (56): 11802-11807.• Zawahir S, Roberts DM, Palangasinghe C, Mohamed F,Eddleston M, Dawson AH, Buckley NA, Ren L, Medley GA,Gawarammana I (2009). Acute intentional self-poisoningwith a herbicide product containing fenoxaprop-P-ethyl,ethoxysulfuron, and isoxadifen ethyl: a prospective observationalstudy. Clin Toxicol 47 (8): 792-797. [CrossRef ]
Birincil Dil
Konular Farmakoloji ve Eczacılık
Yayımlanma Tarihi 10 Ekim 2017
Bölüm Original Article

Yayımlanma Tarihi : 10 Ekim 2017

Makalenin Yazarları
Bahar ULUS