KÜLTÜRÜ YAPILAN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) Staphylococcus hominis subsp. hominis ENFEKSİYONU

Bu çalışmada, ticari bir gökkuşağı alabalığı işletmesinde görülen hastalık çıkışına neden olan patojen bakterilerin örnekleme yapılan balıklardan izole edilerek belirlenmesi ve çeşitli antimikrobiyal maddelere karşı duyarlılıklarının saptanması amaçlanmıştır.Hasta balıklarda; renkte koyulaşma, letarji, gözlerde ekzoftalmus ve hemoraji, ventral yüzgeçlerin dibinde hemoraji, abdominal boşlukta sıvı birikimi ve buna bağlı olarak şişkin karın, hemorajik anüs, splenomegali ve vişne kırmızısı renkte dalak, solgun veya kırmızımsı renge sahip karaciğer ve bağırsaklarda sarımsı mukoid sıvı toplanması gözlenmiştir.Çeşitli biyokimyasal testlerin ve API-Staph ve API-Zym tanı kitlerinin kullanıldığı testler sonucunda, hasta gökkuşağı alabalıklarından izole edilen suşun Staphylococcus hominis subsp. hominis olduğu saptanmıştır. Antibiyotik duyarlılık testinde tanımlanan suşun: ampisilin, basitrasin kanamisin neomisin, oksitetrasiklin polimiksin B streptomisin,  sülfametoksazol/trimetoprim 19:1 antibiyotiklerine duyarlı; sefotaksim, kloramfenikol, eritromisin, flumekuin ve furazolidon antibiyotiklerine dirençli olduğu bulunmuştur.

A Staphylococcus hominis subsp. hominis INFECTION IN CULTURED RAINBOW TROUT (Oncorhynchus mykiss)

The aim of this study was to identify the causative agent of a disease outbreak which occured in a commercial rainbow trout farm in Turkey and to determine antibiotic susceptibility of the isolated strains. Moribund fish showed at least one of the symptoms such as darkening color, lethargy, exophthalmos and focal hemorrhages in the eyes, hemorrhages at the base of the fins, abdominal distension due to ascitic fluid, hemorrhagic anus, splenomegaly and cherry reddish spleen, pale or reddish liver and yellowish mucoid liquid in the intestine. After performing various biochemical tests and using API-Staph identification system, the isolated strain was identified as Staphylococcus hominis subsp. hominis. Antibiotic susceptibility test showed that identified strain was sensitive to ampicillin, bacitracin, kanamycin, neomycin, oxytetracycline, polymyxin B, streptomycin, sulphamethoxazole/ trimethoprim 19:1 and was resistant to cefotaxime, chloramphenicol, erythromycin, flumequine, furazolidone antibiotics.

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