Gökkuşağı Alabalığında (Oncorhynchus mykiss) Ovaryum Sıvısının Sperm Motilite Parametreleri ve Döllenme Üzerindeki Etkisi

Bu çalışmada, Gökkuşağı alabalığında (Oncorhynchus mykiss) sperm aktivasyonunda aktivasyon solüsyonu olarak kuluçkahane suyuna ilaveten ovaryum sıvısının kullanılmasının motilite (%) ve eğrisel hız (VCl, µm/saniye) üzerindeki etkisi ve ovaryum sıvısı uzaklaştırılmış yumurta örneklerinde döllenme yüzdesi araştırılmıştır. Çalışmada üreme döneminde 3+ yaş grubuna ait üç adet dişi ve altı adet erkek balık kullanılmıştır. Her bir dişe bireye ait yumurta örneklerinde, analizi yapılan sperm örnekleri ile hem ovaryum sıvısı muhafaza edilerek hem de uzaklaştırılarak dölleme gerçekleştirilmiştir. Sperm örneklerine ait motilite (%) değerlerinde kuluçka suyu kullanılarak aktive edilmiş örnekler ile ovaryum sıvısı kullanılarak elde edilmiş örnekler arasındaki sonuçlar istatiksel olarak farklı bulunmuşken (p<0.05); motiliteye ait kinematik parametreler arasındaki fark önemsiz bulunmuştur (p˃0.05). Dölleme grupları arasında ise döllenme yüzdesinde herhangi bir fark bulunmamıştır.

Anahtar Kelimeler:

Ovaryum sıvısı, Motilite parametreleri, Gökkuşağı Alabalığı, Döllenme Yüzdesi

The Effect of Ovarian Fluid on Motility Characteristics and Fertilization Ratio of Rainbow Trout (Onchorhynchus mykiss) Sperm

In this study, the effect of ovarian fluid and hatchery water as activation solutions for sperm activation in rainbow trout (Oncorhynchus mykiss) on motility (%) curvilinear velocity (VCl, µm / sec) and fertilization rate were analyzed. Three female and six male fish belonging to the 3+ age group were used in the study. Each females' egg was fertilized with analyzed sperm samples both either removing ovarian fluid or not. Between results of the motility (%) values of sperm samples that activated with hatchery water and ovarian fluid were found statistically different (p <0.05); the difference between the kinematic parameters of motility was insignificant (p˃0.05) among group. There was no difference between the fertilization rate in the groups.

Keywords:

Ovarian fluid, Motility Characteristics, Rainbow trout, fertilization rate,

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