Farklı Çözgenlerle Elde Edilmiş Ulva lactuca Ekstraktlarının Antibakteriyal Aktivitesi

Bu çalışmada, Ulva lactuca ekstraktlarının Gram pozitif (Staphylococus aureus ve Bacilus cereus) ve Gram negatif (Esherichia coli, Eshericha coli O157:H7, Salmonella enteritis, Vibrio parahaemolyticus, Aeomonas hydrophila) bakterilere karşı antibakteriyel aktivitesinin belirlenmesi amaçlanmıştır. Bu amaçla, U. lactuca, aseton, etanol, metanol, hekzan, klorofom ve distile su gibi farklı çözücüler ile ekstrakte edilmiştir. Antibakteriyel aktivitenin belirlenmesi amacıyla disk diffuzyon metodu kullanılmıştır. Araştırma sonunda, en yüksek antibakteriyel aktivite etanol ekstresinde saptanmıştır. Gram pozitif bakterilerin Gram negatif bakterilere kıyasla daha duyarlı olduğu belirlenmiştir. En yüksek inhibisyon zonu S. aureus (26 mm) ve B. cereus’ta (21 mm) tespit edilmiştir. Kloroform ekstresinin E. coli ve A. hydrophila’ya, hekzan ekstresinin ise S. enteritidis ve S. aureus’a karşı orta düzeyde antibakteriyel etki gösterdiği tespit edilmiştir. Aseton ve su ekstrelerinin ise test edilen bakteri türlerine karşı etkili olmadığı saptanmıştır. Bu bulgular, U. lactuca’nın etanol ekstresinin, gıda ve farmasötik endüstrisinde doğal koruyucu madde olarak kullanılma potansiyeline sahip olduğunu göstermiştir.

Anahtar Kelimeler:

alg ekstraktı, disk difüzyon yöntemi, Antibakteriyel aktivite

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